Presentation

The histology platform of the Human Nutrition Unit is located at the INRAE Theix Center, Building “Pariou”, Level 2.

Its aims are:
•    to carry out histology projects, from sample preparation to sectioning and analysis of observations;
•    to pool technical resources and monitor their proper operation;
•    to train/assist people in the techniques and equipment used on the platform.

The platform is open to all INRAE staff, as well as to external personnel for collaborative projects or consulting services. It offers free access to equipment after training and reservation.

Equipement /Expertise

Cryosection :

• 1 cryostat Leica CM950

Paraffin section :

• 1 microtome Leica RM2165
• 1 straightener for spreading on blade

Sample preparation: fixation, dehydration, paraffin embedding

• 1sample preparation station HistoCore Pearl
• 1 hot-mix asphalt plant Leica HistoCore Arcadia H and its cold plate

Imagerie : 1 microscope Olympus statif reverse model IX73P1F team :   

• 1 ultra-sensitive monochrome camera CAM HAMAMATSU FLASH 4.0 LT
• 1 color camera DP23                                                               
• 1 3-color LED source PE300 COOLLED PE300                   
• 4 filtersFITC CY3 Cy5 Dapi                                                           
• 5 lens 4x ; 10x ; 20X ; 40X                                                     
• 1 motorized turntable XYZ                                                               
• 1 control software Cell sens Dimension with stage modul /well navigator 

Identification of samples

• 1 blade printer Epredia SlideMate
• 1 cassette printer Thermo PRINTMATE AS 450

Staining, immunolabeling, image analysis